Is there any difference between actins from intact and denervated muscles?
نویسندگان
چکیده
Various techniques have been used (UV absorbance and fluorescence spectroscopy, polarised UV fluorescence microscopy, viscosimetry, electrophoresis and isoelectric focusing, superprecipitation, etc.) As suggested by several parameters, denervation interferes with the structure of actin by producing local conformation changes in the region of aromatic residues near the sites responsible for actin-actin and actin-myosin interactions.
منابع مشابه
Absence of caspase-3 protects against denervation-induced skeletal muscle atrophy.
The ubiquitin-proteasome system is a key proteolytic pathway activated during skeletal muscle atrophy. The proteasome, however, cannot degrade intact myofibrils or actinomyosin complexes. In rodent models of diabetes mellitus and uremia, caspase-3 is involved in actinomyosin cleavage, generating fragments that subsequently undergo ubiquitin-proteasome-mediated degradation. Here, we demonstrate ...
متن کاملLipoprotein lipase activity in skeletal muscles of the rat: effects of denervation and tenotomy.
The effects of denervation, tenotomy, or tenotomy with simultaneous denervation on the activity of heparin-releasable and intracellular, residual lipoprotein lipase (LPL) and triacylglycerol (TG) content were examined in rat skeletal muscles. An influence of muscle electrostimulation on denervated and tenotomized muscles was also evaluated. Activity of both LPL fractions was decreased in denerv...
متن کاملStudy of the State of F-Actin in Denervated Muscle Fibre by Polarised UV Fluorescence Microscopy
In order to establish the effect of denervation on the structure of F-actin, polarisation of fluorescence of tryptophan residues of F-actin was determined in intact and denervated ghost muscle fibres (freed from myosin, troponin and tropomyosin) under various conditions. P\\, the polarisation parameter obtained by excitation of the fluorescence of tryptophan residues of F-actin by light polaris...
متن کاملDevelopmentally regulated expression of cytochrome-c oxidase isoforms in regenerating rat skeletal muscle.
The developmental expression of tissue-specific isoforms of cytochrome-c oxidase (COX) subunit VIII [heart (COX VIII-H) and liver (COX VIII-L)] and the influence of innervation were examined in regenerating fast [extensor digitorum longus (EDL)] and slow (soleus) muscles. In adult muscles, COX VIII-H was the predominant isoform. The COX VIII-L mRNA was expressed 3 days after induction of regene...
متن کاملUnexpected Fascicle Length Changes In Denervated Feline Soleus Muscle During Stance Phase Of Walking
After surgical repair of traumatically severed peripheral nerves, associated muscles are paralyzed for weeks. Little is known about fascicle length changes in paralyzed muscles during locomotion. The aim of this study was to investigate to what extent, if any, muscle fascicles of denervated feline soleus (SO) change length during stance of walking when intact SO synergists are actively contract...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- General physiology and biophysics
دوره 6 3 شماره
صفحات -
تاریخ انتشار 1987